column,4.0
gst,4.0
protein,4.0
bcl-gl,3.0
protease,3.0
ph,3.0
tris-hcl,3.0
nacl,3.0
β-mercaptoethanol,2.0
according,2.0
healthcare,2.0
performed,2.0
remove,2.0
dialyzed,2.0
supplier,2.0
ge,2.0
overnight,2.0
applied,2.0
sv40,2.0
glutathione,2.0
affinity,2.0
recombinant,2.0
using,2.0
his-tag,2.0
st,2.0
ni-nta,2.0
tev,2.0
free,1.0
gst-fused,1.0
confirmation,1.0
4b,1.0
purification,1.0
bl21,1.0
complexes,1.0
reapplied,1.0
escherichia,1.0
cleavage,1.0
instructions,1.0
ril,1.0
beads,1.0
s-transferase,1.0
target,1.0
under,1.0
from,1.0
prescission,1.0
filters,1.0
imidazole,1.0
stratagene,1.0
subunit,1.0
cleaved,1.0
flow-through,1.0
eluted,1.0
coli,1.0
competent,1.0
gst-tag,1.0
direct,1.0
instructionssoluble,1.0
buffer,1.0
removed,1.0
proteins,1.0
sepharose,1.0
nondenaturing,1.0
dtt,1.0
syringe,1.0
pre-equilibrated,1.0
codon-plus,1.0
fractions,1.0
melk,1.0
elution,1.0
strain,1.0
on-column,1.0
binding,1.0
expressed,1.0
filtered,1.0
fraction,1.0
conditions,1.0
cells,1.0
