arabidopsis
leaves
fixed
solution
v/v
paraformaldehyde
v/v
glutaradehyde
phosphate
buffer
ph
min
under
vacuum
room
temperature
specimens
dehydrated
graded
ethanol
series
infiltrated
stepwise
increments
lr
white
resin
london
resin
theale
berkshire
uk
polymerized
h.
materials
immuno-gold
labelling
prepared
according
procedure
varagona
raikhel
modification
described
specimens
nm
sectioned
using
leica
reichert
ultracut
microtome
mounted
formvar-coated
slotted
grids
grids
incubated
blocking
solution
ttbs
containing
w/v
fish
skin
gelatin
w/v
bsa
min
anti-acbp4
antibodies
diluted
blocking
solution
added
incubated
room
temperature
h.
grids
rinsed
three
times
each
min
ttbs
incubated
nm
gold-conjugated
goat
anti-rabbit
igg
secondary
antibody
sigma
diluted
blocking
solution
grids
rinsed
three
times
each
min
ttbs
following
three
5-min
rinses
distilled
water
stained
w/v
uranyl
acetate
min
followed
w/v
lead
citrate
min
sections
visualized
photographed
using
philips
em208s
electron
microscope
operating
kv
controls
performed
excluding
primary
antibody
immuno-gold
labelling
acbp4
arabidopsis
leaf
cell
using
transmission
electron
microscopy
transverse
sections
stained
affinity-purified
acbp4-specific
antibodies
transverse
sections
leaves
stained
acbp4-specific
antibodies
magnification
boxed
area
control
labelling
leaf
cell
using
secondary
antibodies
alone
arrowheads
gold
particles
vacuole
cytosol
ch
chloroplast
nucleus
cw
cell
wall
bars
represent
immuno-electron
microscopy
carried
out
using
transverse
sections
leaves
2-week-old
arabidopsis
germinated
grown
ms
medium
under
light/dark
regime
although
immuno-gold
labelling
anti-acbp4
antibodies
mostly
evident
cytosol
signals
detected
periphery
nucleus
fig.
3b
control
primary
antibody
replaced
blocking
solution
significant
immuno-gold
labelling
observed
fig.
3d
immunolocalization
signals
periphery
nucleus
may
culminated
from
interaction
acbp4
atebp
study
gfp
atebp
confined
nucleus
also
detected
cytosol
interact
acbp4
acbp4
dsred
transiently-expressed
tobacco
leaves
predominantly
targeted
cytosol
immuno-electron
microscopy
indicated
localization
acbp4
cytosol
signals
detected
periphery
nucleus
perhaps
consequence
interaction
atebp