map
phosphorylation
site
systematically
decided
peptide
array
displays
peptides
containing
serine
threonine
residues
ddx3x
supplementary
figure
incubation
peptide
array
purified
tbk1
produced
number
phosphorylation
signals
figure
7a
tbk1
control
peptide
phosphorylated
tbk1
suggesting
activation
loop
indeed
autophosphorylation
site
analysing
remaining
hits
peptide
array
identified
potential
phosphorylation
sites
ddx3x-derived
peptides
figure
7a
four
target
sites
s181
s183
s240
s269
found
dead
domain
contains
atpase
activity
ddx3x
seven
remaining
sites
scattered
throughout
helicase
domain
s429
t438
s442
s456
s520
t542
s543
used
information
gathered
peptide
array
derive
tbk1
consensus
phosphorylation
site
figure
7b
strikingly
tbk1
showed
strong
preference
serine
over
threonine
fact
none
peptides
displayed
array
contain
exclusively
threonines
phosphorylated
mapping
tbk1
phosphorylation
site
ddx3x
peptides
derived
from
ddx3x
control
peptides
derived
from
tbk1
irf3
along
alanine
mutants
incubated
tbk1
presence
γ-32p
atp
each
array
contained
total
peptides
spotted
triplicate
phosphorylation
sites
obtained
from
peptide
array
see
also
text
used
build
tbk1
phosphorylation
consensus
sequence