identify
novel
interactors
tbk1
generated
stable
raw264
.7
cell
lines
express
gs-tap-tagged
version
tbk1
purified
tbk1
protein
complex
tandem
affinity
purification
figure
1a
burckstummer
et
al
gs-tap-tagged
ddx3x
isolated
affinity
purification
using
rabbit
igg
agarose
also
recruited
enhanceosome
region
upon
l.
monocytogenes
infection
figure
5c
recruitment
specific
control
region
figure
5a
amplified
pcr
under
conditions
suggests
ddx3x
exerts
direct
effect
ifn-β
promoter
tandem
affinity
purification
using
gs-tap
cassette
performed
described
previously
burckstummer
et
al
dead-box
helicase
ddx3x
target
tbk1
schematic
representation
tandem
affinity
purification
protocol
gs-tap-tagged
tbk1
expressed
raw264
.7
cells
purified
using
rabbit
immunoglobulin
igg
agarose
eluted
tobacco
etch
virus
tev
protease
cleavage
next
remaining
complex
purified
using
streptavidin
agarose
eluted
boiling
sds
sample
buffer
final
tap
eluate
separated
sds
page
stained
silver
staining
protein
complex
composition
analysed
lc-msms
position
several
complex
components
depicted
next
region
identified