gst,5.0
proteins,5.0
protein,5.0
recombinant,5.0
human,5.0
expressed,5.0
column,4.0
purified,4.0
coli,4.0
affinity,4.0
bcl-gl,3.0
bl21,3.0
appl1,3.0
protease,3.0
ph,3.0
tris-hcl,3.0
using,3.0
nacl,3.0
cells,3.0
β-mercaptoethanol,2.0
chromatography,2.0
e.,2.0
according,2.0
healthcare,2.0
escherichia,2.0
performed,2.0
beads,2.0
genbank,2.0
n-terminal,2.0
remove,2.0
id,2.0
dialyzed,2.0
c.,2.0
supplier,2.0
ge,2.0
removed,2.0
overnight,2.0
applied,2.0
residues,2.0
sv40,2.0
glutathione,2.0
tag,2.0
strain,2.0
his-tag,2.0
st,2.0
ni-nta,2.0
tev,2.0
phosphate-buffered,1.0
cases,1.0
variants,1.0
free,1.0
nm_020673,1.0
isopropyl-β-d-thiogalactopyranoside,1.0
gst-fused,1.0
including,1.0
confirmation,1.0
4b,1.0
introduced,1.0
bar-ph,1.0
aggregationsoluble,1.0
fused,1.0
rabaptin5,1.0
purification,1.0
complexes,1.0
his-tagged,1.0
lysed,1.0
reapplied,1.0
v/v,1.0
gst-affinity,1.0
bc021901,1.0
iptg,1.0
cleavage,1.0
instructions,1.0
ril,1.0
s-transferase,1.0
target,1.0
under,1.0
sample,1.0
both,1.0
zhai,1.0
bar,1.0
c734s,1.0
from,1.0
reduce,1.0
saline,1.0
prescission,1.0
invitrogen,1.0
stored,1.0
rab21,1.0
soluble,1.0
filters,1.0
imidazole,1.0
stratagene,1.0
nm_004162,1.0
subunit,1.0
cleaved,1.0
mutations,1.0
lysate,1.0
flow-through,1.0
concentrated,1.0
eluted,1.0
competent,1.0
gst-tag,1.0
direct,1.0
lysozyme,1.0
sigma,1.0
rab22a,1.0
induction,1.0
caa62580,1.0
et,1.0
al,1.0
de3,1.0
additional,1.0
buffer,1.0
mg,1.0
domains,1.0
described,1.0
sepharose,1.0
βme,1.0
two,1.0
nondenaturing,1.0
dtt,1.0
syringe,1.0
his-selecttm,1.0
c719s,1.0
pre-equilibrated,1.0
pbs,1.0
supernatant,1.0
harvested,1.0
codon-plus,1.0
fragments,1.0
fractions,1.0
melk,1.0
elution,1.0
previously,1.0
thrombin,1.0
rab5a,1.0
on-column,1.0
binding,1.0
point,1.0
instructionsrecombinant,1.0
filtered,1.0
fraction,1.0
conditions,1.0
startm,1.0
