column,5.28771237954945
recombinant,5.279266173667587
gst,5.187373527093316
expressed,5.162107388461887
human,5.087437133644205
proteins,5.032131345797165
protein,5.014355179273776
affinity,4.417346639258943
coli,4.417346639258943
bl21,4.378294855911892
purified,4.242166167740204
tris-hcl,3.23400753600382
protease,3.201342587575611
dialyzed,3.1699250014423126
id,3.1699250014423126
nacl,3.118585092559912
bcl-gl,3.0312498478360155
appl1,3.030805006361481
ph,3.0220259260235043
using,3.011315261667626
cells,3.0098031415017323
tag,2.8300749985576874
his-tag,2.643856189774725
β-mercaptoethanol,2.643856189774725
removed,2.5260688116675873
tev,2.444784842672896
ni-nta,2.444784842672896
chromatography,2.444784842672896
remove,2.3398500028846247
escherichia,2.3398500028846247
genbank,2.2750070474998703
supplier,2.1990713471018286
c.,2.1560050240025466
e.,2.1480011628875535
applied,2.140778655782796
overnight,2.140778655782796
beads,2.122801089328287
ge,2.108895568044753
healthcare,2.108895568044753
glutathione,2.0887882387169068
strain,2.086137443783772
n-terminal,2.073051752050228
according,2.0387306497338624
sv40,2.031193710102037
performed,2.028427718439402
residues,2.0189206584981325
st,2.011801202879368
startm,1.5849625007211563
filtered,1.5849625007211563
on-column,1.5849625007211563
rab5a,1.5849625007211563
thrombin,1.5849625007211563
codon-plus,1.5849625007211563
pre-equilibrated,1.5849625007211563
c719s,1.5849625007211563
his-selecttm,1.5849625007211563
syringe,1.5849625007211563
nondenaturing,1.5849625007211563
caa62580,1.5849625007211563
rab22a,1.5849625007211563
lysozyme,1.5849625007211563
gst-tag,1.5849625007211563
competent,1.5849625007211563
flow-through,1.5849625007211563
cleaved,1.5849625007211563
nm_004162,1.5849625007211563
imidazole,1.5849625007211563
prescission,1.5849625007211563
c734s,1.5849625007211563
zhai,1.5849625007211563
ril,1.5849625007211563
bc021901,1.5849625007211563
gst-affinity,1.5849625007211563
reapplied,1.5849625007211563
confirmation,1.5849625007211563
gst-fused,1.5849625007211563
isopropyl-β-d-thiogalactopyranoside,1.5849625007211563
nm_020673,1.5849625007211563
phosphate-buffered,1.5849625007211563
de3,1.3219280948873624
filters,1.3219280948873624
saline,1.3219280948873624
free,1.3219280948873624
elution,1.222392421336448
concentrated,1.222392421336448
stored,1.222392421336448
reduce,1.222392421336448
iptg,1.222392421336448
βme,1.1699250014423124
sepharose,1.1699250014423124
stratagene,1.1375035237499351
cleavage,1.1375035237499351
v/v,1.1154772174199359
rabaptin5,1.0995356735509143
fused,1.0995356735509143
supernatant,1.0780025120012733
mg,1.0780025120012733
soluble,1.0780025120012733
s-transferase,1.0780025120012733
cases,1.0780025120012733
fragments,1.070389327891398
eluted,1.070389327891398
lysate,1.070389327891398
introduced,1.070389327891398
his-tagged,1.0641303374197155
pbs,1.0588936890535687
sigma,1.0544477840223765
4b,1.0544477840223765
fraction,1.0506260730699681
purification,1.0506260730699681
rab21,1.0473057147783569
lysed,1.0473057147783569
variants,1.0395283641866373
dtt,1.037474705418663
additional,1.037474705418663
sample,1.0339473319233377
harvested,1.0324214776923775
fractions,1.0310268956206246
invitrogen,1.0310268956206246
instructions,1.0310268956206246
point,1.029747343394052
direct,1.0230836131130412
target,1.0188590272513158
including,1.0188590272513158
complexes,1.0179219079972623
conditions,1.0166787411466305
induction,1.0163018123291006
mutations,1.0159415438690216
bar-ph,1.0140751852117227
buffer,1.0128240403575837
described,1.0110551885089958
domains,1.0092185300293788
previously,1.0086648611271276
bar,1.0079051980853462
melk,1.007417471562483
two,1.006542845866772
subunit,1.005900601439684
both,1.0055810322632999
under,1.0053334443030408
al,1.0047379190066632
binding,1.003657023608089
from,1.0024267373945401
et,1.0024024902153046
instructionsrecombinant,1.0
aggregationsoluble,1.0
