mg,2.0
protein,2.0
expressed,2.0
β-mercaptoethanol,1.0
chromatography,1.0
e.,1.0
novagen,1.0
purification,1.0
v/v,1.0
healthcare,1.0
appl1,1.0
both,1.0
media,1.0
ph,1.0
stored,1.0
supplemented,1.0
tris,1.0
purified,1.0
concentrated,1.0
similarly,1.0
coli,1.0
plyss,1.0
sigma,1.0
b834,1.0
resource-qtm,1.0
de3,1.0
ge,1.0
proteins,1.0
procedure,1.0
βme,1.0
samples,1.0
native,1.0
se-met,1.0
using,1.0
minimal,1.0
needed,1.0
se-met-substituted,1.0
mutants,1.0
anion-exchange,1.0
cells,1.0
hcl,1.0
