purification
resource-qtm
anion-exchange
chromatography
ge
healthcare
both
protein
samples
concentrated
mg
tris
hcl
ph
v/v
β-mercaptoethanol
βme
stored
needed
appl1
mutants
expressed
similarly
se-met-substituted
proteins
expressed
e.
coli
b834
de3
plyss
cells
novagen
minimal
media
supplemented
mg
se-met
sigma
purified
using
procedure
native
protein