soluble
fractions
cell
lysate
containing
his-tagged
appl1
variants
analyzed
sds
page
confirm
comparable
expression
level
solubility
gsh
resin
carrying
nucleotide-loaded
gst
rab
fusion
protein
incubated
cell
lysate
appl1
variant
kda
min
washed
three
times
pbs
dtt
mgcl2
resuspended
reducing
sds
sample
buffer
sample
subjected
sds
page
analysis
visualized
coomassie
blue
stain