The effects of serotonin and its antagonists on slowly adapting type I mechanoreceptive fibers in frog skin
| dc.contributor | Graduate Program in Biomedical Engineering. | |
| dc.contributor.advisor | Güçlü, Burak. | |
| dc.contributor.author | Mutlu, Sevinç. | |
| dc.date.accessioned | 2023-03-16T13:12:10Z | |
| dc.date.available | 2023-03-16T13:12:10Z | |
| dc.date.issued | 2009. | |
| dc.description.abstract | It was intended to investigate serotonin as the neurotransmitter between Merkel cell and its nerve ending, through changes in SA-I response to exogenically applied serotonin and its antagonists. Single-unit electrophysiological responses were recorded from the dorsal cutaneous nerves of the common water frog's (Rana ridibunda) skin perfused in a tissue bath. Maintained mechanical stimulation was applied at suprathreshold levels by von Frey hairs calibrated at 0.16, 0.4, 1, 1.4, and 2 g-bending forces. The effect of serotonin was tested at concentrations: 10 μM (n=8), 100 μM (n=7), 1000 μM (n=6). The responses were analyzed as spike rates. Paired-t test was used to test the significance of the results. The spike rate increased as a linear function of the stimulus level at baseline and all tested concentrations. Additionally spike rate increased significantly at 10 μM (p<0.05) and at 100 μM (p<0.05), but decreased at 1000 μM (p<0.05) compared to the baseline. In order to find out which serotonin receptors are involved in the process, selective 5-HT3 (n=7) and 5-HT2 (n=6) receptor antagonists were applied at 100 μM concentrations. Both receptor antagonists decreased SA-I responses(p<0.05). Recovery was obtained at each case other than 1000 μM serotonin application. Our results confirm the role of serotonin in the mechanoelectric transduction in Merkel cell-nerve ending complex and that at least two serotonin receptors are involved in the process in frog skin.|Keywords: Somatosensory, Tactile fiber, Cutaneous afferent, Mechanoreceptor. | |
| dc.format.extent | 30cm. | |
| dc.format.pages | xiv, 59 leaves; | |
| dc.identifier.other | BM 2009 M88 | |
| dc.identifier.uri | https://digitalarchive.library.bogazici.edu.tr/handle/123456789/18777 | |
| dc.publisher | Thesis (M.S.)-Bogazici University. Institute of Biomedical Engineering, 2009. | |
| dc.relation | Includes appendices. | |
| dc.relation | Includes appendices. | |
| dc.subject.lcsh | Somatosensory evoked potentials. | |
| dc.subject.lcsh | Tactile sensors. | |
| dc.subject.lcsh | Mechanoreceptors. | |
| dc.subject.lcsh | Afferent pathways. | |
| dc.title | The effects of serotonin and its antagonists on slowly adapting type I mechanoreceptive fibers in frog skin |
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