Fabrication of thiol reactive polymeric microstructures

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dc.contributorGraduate Program in Chemistry.
dc.contributor.advisorSanyal, Amitav.
dc.contributor.authorGevrek, Tuğçe Nihal.
dc.date.accessioned2023-03-16T11:01:44Z
dc.date.available2023-03-16T11:01:44Z
dc.date.issued2011.
dc.description.abstractPEG-based 3D hydrogel patterns containing thiol reactive maleimide functional groups have been prepared using a novel Diels-Alder/retro Diels-Alder strategy. Bulk-hydrogels and hydrogel patterns containing various amount of thiol reactive maleimide were synthesized by photo-polymerization in presence of photoinitiator and PEG-DA cross linker. The thiol reactive maleimide group was protected with furan during gelation of bulk-hydrogel and patterning of hydrogels and then was deprotected by retro-Diels-Alder reaction. After activation of maleimide in the hydrogel structure, the reactive maleimide group embedded hydrogels can be efficiently derivatized with thiol containing molecules such as a fluorescent dye, BodipyC10SH. Thiol containing biotin derivatives were covalently attached to the thiol reactive maleimide in bulk-hydrogel and hydrogel patterns under mild condition. Immobilization of FITC-streptavidin onto biotinylated hydrogel patterns were performed and investigated by fluorescence microscope. In order to detect amount of accessible biotin in the hydrogel, HABA-assay was carried out with biotinylated bulk-hydrogels.
dc.format.extent30 cm.
dc.format.pagesxii, 44 leaves ;
dc.identifier.otherCHEM 2011 G48
dc.identifier.urihttps://digitalarchive.library.bogazici.edu.tr/handle/123456789/14485
dc.publisherThesis (M.S.)-Bogazici University. Institute for Graduate Studies in Science and Engineering, 2011.
dc.relationIncludes appendices.
dc.relationIncludes appendices.
dc.subject.lcshThiols.
dc.subject.lcshReactive polymers.
dc.titleFabrication of thiol reactive polymeric microstructures

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