Fluorescence lifetime images of dye molecules

Abstract

In this thesis, Fluorescence Lifetime Images of perylene and bodipy dye molecules mixture are studied using a time-correlated single photon-counting technique. Lifetimes of dye molecules are calculated pixel-by-pixel from these time-resolved images, and the spatial variations of the lifetimes are then displayed in a pseudocolor format. Three images with resolutions 77 x 39 ¹m2, 9.7 x 9.7 ¹m2 and 7.7 x 7.7 ¹m2 are obtained, lifetimes are changing from 4.78 ns to 5.38 ns, from 4.78 ns to 4.98 ns and from 4.78 ns to 4.98 ns, respectively.